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KMID : 0869120100120020073
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기초간호자연과학회지
2010 Volume.12 No. 2 p.73 ~ p.80
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In vitro Inhibitory Effect of Alcohol Hand Rubs Against Staphylococcus aureus
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Moon Cheol
Kim Gyeong-Mi
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Abstract
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Purpose: We tested the inhibitory effect of six purchasable alcohol hand rubs against Staphylococcus aureus.
Methods: Five alcohol hand rubs were collected with random manner on the market and 1 alcohol hand rubs which is currently used in a hospital was included. we designed the experimental scheme on the basis of M7-A6 method of NCCLS. Each hand rubs were diluted to 1:1, 1:10, 1:100, 1:1,000 with tryptic soy broth. S. aureus was cultured. Turbidity and the colony counting was measured.
Results: With dilution rate of 1:1, all products showed more than 95% of the inhibitory effect on bacterial growth. At 1:10 dilution, product , 2, 3, 4, and 5 displayed the inhibitory effect of 97.77%, 92.38%, 78.19%, 97.42%, and 96.6%, respectively. But, the inhibitory effect of product has been disappeared at that dilution. Over 1:100 dilution, all products lost their inhibitory effect except product . Product displayed more than 96% of inhibitory effect at all dilution rate, even at 1:100 and 1:1,000.
Conclusion: We identified that inhibitory effect on bacterial growth of alcohol hand rubs was variable. We suggest that concentration of alcohol should be checked before choosing alcohol hand rubs. Further evaluation of in vivo study is needed.
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KEYWORD
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Alcohol, Handwashing, Staphylococcus aureus
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